rabbit anti-hdac2 polyclonal antibody d155199 (Bio Basic Canada)

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Bio Basic Canada rabbit anti-hdac2 polyclonal antibody d155199
The effects of silencing <t>HDAC2</t> or HDAC3 on Ace-H3K9 levels in the promoter region of LPL, miR-29a and PPARγ in SH-SY5Y cells. ChIP-PCR assay was used to measure the levels of Ace-H3K9 in the promoter region of LPL (A) , miR-29a (B) , and PPARγ (C) in cells ( n = 6; mean ± SD; Student’s t -test; ** p < 0.01 vs. scrambled siRNA group).

The effects of silencing <t>HDAC2</t> or HDAC3 on Ace-H3K9 levels in the promoter region of LPL, miR-29a and PPARγ in SH-SY5Y cells. ChIP-PCR assay was used to measure the levels of Ace-H3K9 in the promoter region of LPL (A) , miR-29a (B) , and PPARγ (C) in cells ( n = 6; mean ± SD; Student’s t -test; ** p < 0.01 vs. scrambled siRNA group).

Rabbit Anti Hdac2 Polyclonal Antibody D155199, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-hdac2+polyclonal+antibody+d155199/rabbit+anti+hdac2+polyclonal+antibody+d155199/pmc07235190-42-6-20
Average 90 stars, based on 1 article reviews

rabbit anti-hdac2 polyclonal antibody d155199 - by Bioz Stars, 2026-09

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1) Product Images from "Exploration of the Molecular Mechanism for Lipoprotein Lipase Expression Variations in SH-SY5Y Cells Exposed to Different Doses of Amyloid-Beta Protein"

Article Title: Exploration of the Molecular Mechanism for Lipoprotein Lipase Expression Variations in SH-SY5Y Cells Exposed to Different Doses of Amyloid-Beta Protein

Journal: Frontiers in Aging Neuroscience

doi: 10.3389/fnagi.2020.00132

The effects of silencing HDAC2 or HDAC3 on Ace-H3K9 levels in the promoter region of LPL, miR-29a and PPARγ in SH-SY5Y cells. ChIP-PCR assay was used to measure the levels of Ace-H3K9 in the promoter region of LPL (A) , miR-29a (B) , and PPARγ (C) in cells ( n = 6; mean ± SD; Student’s t -test; ** p < 0.01 vs. scrambled siRNA group).

Figure Legend Snippet: The effects of silencing HDAC2 or HDAC3 on Ace-H3K9 levels in the promoter region of LPL, miR-29a and PPARγ in SH-SY5Y cells. ChIP-PCR assay was used to measure the levels of Ace-H3K9 in the promoter region of LPL (A) , miR-29a (B) , and PPARγ (C) in cells ( n = 6; mean ± SD; Student’s t -test; ** p < 0.01 vs. scrambled siRNA group).

Techniques Used:

Alterations of PPARγ expression in SH-SY5Y cells with different treatments. The relative expression of PPARγ mRNA and protein were respectively analyzed by qRT-PCR and Western blot in cells exposed to 2 or 10 μM amyloid-β (Aβ; A–C ), with HDAC2/3-silencing treatment (D–I) , and treated with miR-29a mimic or inhibitor [ J,K ; n = 6; mean ± SD; One-way ANOVA followed by LSD multiple comparison tests, Student’s t -test; ** p < 0.01 vs. control (A,C) , scrambled siRNA (D,F,G,I) or mimic control (J,L) , ## p < 0.01 vs. inhibitor control].

Figure Legend Snippet: Alterations of PPARγ expression in SH-SY5Y cells with different treatments. The relative expression of PPARγ mRNA and protein were respectively analyzed by qRT-PCR and Western blot in cells exposed to 2 or 10 μM amyloid-β (Aβ; A–C ), with HDAC2/3-silencing treatment (D–I) , and treated with miR-29a mimic or inhibitor [ J,K ; n = 6; mean ± SD; One-way ANOVA followed by LSD multiple comparison tests, Student’s t -test; ** p < 0.01 vs. control (A,C) , scrambled siRNA (D,F,G,I) or mimic control (J,L) , ## p < 0.01 vs. inhibitor control].

Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Comparison, Control

Dose-effect relationships between Aβ concentration and HDAC2/3 expression levels in SH-SY5Y cells. The relative expression of HDAC2 and HDAC3 mRNA (A,B) and protein (C–E) were analyzed by qRT-PCR and Western blot, respectively. β-actin was used as a reference standard [ n = 6; mean ± SD; One-way ANOVA followed by LSD multiple comparison tests; ** p < 0.01 vs. control (0 μm Aβ); # p < 0.05, ## p < 0.01 vs. 2.5 μm Aβ; Δ p < 0.05, ΔΔ p < 0.01 vs. 5 μm Aβ; & p < 0.05, && p < 0.01 vs. 7.5 μm Aβ].

Figure Legend Snippet: Dose-effect relationships between Aβ concentration and HDAC2/3 expression levels in SH-SY5Y cells. The relative expression of HDAC2 and HDAC3 mRNA (A,B) and protein (C–E) were analyzed by qRT-PCR and Western blot, respectively. β-actin was used as a reference standard [ n = 6; mean ± SD; One-way ANOVA followed by LSD multiple comparison tests; ** p < 0.01 vs. control (0 μm Aβ); # p < 0.05, ## p < 0.01 vs. 2.5 μm Aβ; Δ p < 0.05, ΔΔ p < 0.01 vs. 5 μm Aβ; & p < 0.05, && p < 0.01 vs. 7.5 μm Aβ].

Techniques Used: Concentration Assay, Expressing, Quantitative RT-PCR, Western Blot, Comparison, Control

The regulatory mechanism of HDAC2/3 on LPL expression in SH-SY5Y cells. HDAC2 down-regulates microRNA-29a expression by decreasing the level of histone acetylation in its promoter region, subsequently increasing LPL expression directly or through the PPARγ-LPL pathway. HDAC3 decreases LPL expression through inhibiting histone acetylation levels in the promoter region of LPL and PPARγ and up-regulating miR-29a expression which the level of histone acetylation in its promoter region is unaltered.

Figure Legend Snippet: The regulatory mechanism of HDAC2/3 on LPL expression in SH-SY5Y cells. HDAC2 down-regulates microRNA-29a expression by decreasing the level of histone acetylation in its promoter region, subsequently increasing LPL expression directly or through the PPARγ-LPL pathway. HDAC3 decreases LPL expression through inhibiting histone acetylation levels in the promoter region of LPL and PPARγ and up-regulating miR-29a expression which the level of histone acetylation in its promoter region is unaltered.

Techniques Used: Expressing

Expressing:

Article Title: Exploration of the Molecular Mechanism for Lipoprotein Lipase Expression Variations in SH-SY5Y Cells Exposed to Different Doses of Amyloid-Beta Protein
Article Snippet: The rabbit anti-PPARγ polyclonal antibody (D262458), rabbit anti-HDAC2 polyclonal antibody (D155199), and rabbit anti-HDAC3 polyclonal antibody (D260481) were purchased from Bio Basic Inc. (Canada); rabbit anti-LPL polyclonal antibody (sc-32885) and rabbit anti-β-actin polyclonal antibody (sc-130656) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Goat anti-rabbit immunoglobulin G (lgG) secondary antibody was from Shanghai Sangon Biotech Company Limited (D111018, Shanghai, China).

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rabbit anti-hdac2 polyclonal antibody d155199 (Bio Basic Canada)

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